By Sterling Chaykin (auth.), Seymour Rothchild (eds.)
Radioactive tracers have had a profound effect on medical learn in past times 20 years, really in bio chemistry and allied fields. The fast evolution of subtle instrumentation and labeling recommendations up to now ten years has made attainable on a regimen foundation exquisitly delicate experiments which with different suggestions will require her culean efforts at the a part of the investigator. The Symposia on Advances in Tracer technique, which were held on a regular basis on account that 1957, offer for study staff in biochemistry, pharmacology, endocrinology, and medication a spotlight at the quite a few advancements within the tracer box which are often scattered through the medical literature. quantity three is arecord of the lawsuits of the 9th and 10th Symposia, that have been backed by way of the hot England Nuclear company and the Packard device corporation, Inc. fresh advances in tracer method, as mirrored via the papers during this quantity, were quite often interested by im proved analytical innovations, equivalent to liquid scintillation count number ing, autoradiography, and chromatography, and with novel ap plications of categorized compounds in biochemical and medical problems.
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Extra info for Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology
3) according to the method of Straub  proved that the pharmacological 35 Georg Rabitzsch Fig. 5. Activity distribution of the thin layer chrotnatography (silica gel G, ethyl acetate/methanol, SbCl s) of digitoxin-23-C14 (right). effect was identical to that of natural digitOJun by the appearance of the positive inotropic effect after 5 p_g and the raising of the basis line, with subsequent systolic heart stoppage, after 10 fl-g. The course of the reaction and the end-product were examined by thin layer chromatography in various systems and by application of several sensitive color reactions 6].
00 Yield of esterification . . • . . • . . . . . . . . . . . . 80 to 90"/0 3H-recovery through the total analysis . . . . . . . . . . . 10 to 15"lo Chrom atogra ms: 1. Reversed phase system 2. Thin layer formamide 3. Thin layer formamide (oxidized ester) 4. Thin layer (2,4-dinitro-phenylhydrazone of oxidized ester) 53 L. Thuneberg Table Il. 0 are subjected to repeated chromatographic purification. The 3H_ and 35S-activities are finally measured together with the proper 3H_ and 35S-standards.
6 6-aminopurine (adenine) . •• 110 mc mc mc mc mc per per per per per millimole millimole millimole millimole milli mole We observe in this series some conspicuous peculiarities. First. one would expect the substituent-free purine to show the highest tritium incorporation because of the highest number of CH-groups which can be labeled. Actually. however, the individual substituents affect the basic structure, causing a decrease or increase of substitution which is dependent on the site of the substitution.