By Alfred Brown, Heidi Smith

Benson's Microbiological Applications has been the ultimate of microbiology lab manuals for over 30 years. The self-contained, clearly-illustrated workouts and four-color layout makes this the appropriate lab guide. acceptable for both a majors or non-majors lab direction, this guide assumes no earlier natural chemistry has been taken.

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Your instructor will indicate the number of identifications that are to be made. 5 1. Penicillium—bluish-green; brush arrangement of phialospores. 2. Aspergillus—bluish-green with sulfur-yellow areas on the surface. Aspergillus niger is black. 3. Verticillium—pinkish-brown, elliptical microconidia. 4. Trichoderma—green, resemble Penicillium macroscopically. 5. Gliocadium—dark green; conidia (phialospores) borne on phialides, similar to Penicillium; grows faster than Penicillium. 6. Cladosporium (Hormodendrum)—light green to grayish surface; gray to black back surface; blastoconidia.

Aseptic Invertebrates Aseptic Technique 10. 4), allow to cool, and place the loop in its container. 11. Incubate the inoculated agar slant at 30° C for 24–48 hours. WORKING WITH AGAR PLATES (Inoculating a slant from a Petri plate) The transfer of organisms from colonies on agar plates to slants or broth tubes is very similar to the procedures used in the last two transfers (broth to broth and slant to slant). The following rules should be observed. Loops vs. Needles In some cases a loop is used.

1 • Exercise 6 Protozoans 27 Benson: Microbiological Applications Lab Manual, Eighth Edition Exercise 6 • II. Survey of Microorganisms 6. Protozoa, Algae, and Cyanobacteria Protozoa, Algae, and Cyanobacteria Phylum Sarcomastigophora Members of this phylum have been subdivided into two subphyla: Sarcodina and Mastigophora. Sarcodina (Amoebae) Members of this subphylum move about by the formation of flowing protoplasmic projections called pseudopodia. The formation of pseudopodia is commonly referred to as amoeboid movement.

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