By Sukriti Nag
Univ. of Toronto, Canada. textual content presents the main points of chosen morphologic, permeability, delivery, in vitro, and molecular ideas for BBB reports. every one half is preceded via a assessment emphasizing the benefits and pitfalls of specific recommendations. Written for researchers in BBB. DNLM: Blood--Brain Barrier--physiology.
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Additional resources for Blood'Brain Barrier: Biology and Research Protocols
B. Wash slide with distilled water and dip in acetone to differentiate. c. Dehydrate in 2 changes of 100% ethanol and then in 2 changes of xylene for 2 min each. d. Place a drop of Permount on the section and then a cover slip. 2. Ultrathin Sectioning Ultrathin sections are cut from a preselected area of the block, usually based on information obtained by viewing the semithin section. Most sections within the silver to pale gold range (60–90 nm) are suitable for normal work, although thinner sections may be required when high resolution is needed.
The method for manual staining of ultrathin sections is as follows: 1. If en bloc staining with uranyl acetate is done proceed to lead citrate staining (step 4). 2. Place a drop of uranyl acetate on a silicone rubber plate contained in a petri dish. Float the grid on this drop with the section side down for 20 min. Because the surface of this rubber plate is divided into numbered squares, four to five sections can be stained at a time. 3. Rinse the grid by 10–15 quick dips in a 250-mL beaker containing filtered water.
1987) Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells. J. Cell Biol. 105, 1455–1462. 249. , and Sage, E. H. (1998) Transforming growth factor-beta1 induces apoptotic death in cultured retinal endothelial cells but not in pericytes: Association with decreased expression of p21waf1/cip1. J. Cell Biochem. 70, 70–83. 250. Hirshi, K. , and D′Amore, P. A. (1997) Control of angiogenesis by pericytes: molecular mechnaisms and significance. , 79, 419–428. 251. , et al.