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3 Organization Within snRNA Gene Loci In the case of the human V1 genes, restriction mapping and direct nucleotide sequence analyses of ten independent isolates revealed that the sequences flanking the V 1 coding regions are identical for several hundred base pairs upstream (Manser and Gesteland 1982; Lund and Dahlberg 1984; Bernstein et al. 1985) and are surprisingly homologous over large distances (Manser and Gesteland 1982; Htun et al. 1984); surprisingly, the homologous sequences extend for more than 20 kb in both directions (Bernstein et al.

Eur J Biochem 99:447-456 Ochs R, Lischwe M, Spohn WH, Busch H (1985) Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. 8S) nuclear RNA. Nucl Acids Res 9:5145-5158 Parker K, Steitz JA (1987) Structural studies of the human U3 ribonucleoprotein reveal a conserved sequence available for base-pairing with a pre-ribosomal RNA. Mol Cell BioI 7:2899-2913 Parker R, Siliciano PG, Guthrie C (1987) Recognition of TACTAAC box during mRNA splicing in yeast involves base-pairing to the U2-like snRNA.

J BioI Chem 255:8901-8906 Epstein P, Reddy R, Busch H (1981) Site-specific cleavage by T1 RNase of U1 RNA in U1 ribonucleoprotein particles. Proc Natl Acad Sci USA 78:1562-1566 Epstein P, Reddy R, Busch H (1984) Multiple states of U3 RNA in Novikoff hepatoma cells. Biochemistry 23:5421- 5425 Forbes D, Kirschner M, Caput D, Dahlberg J, Lund E (1984) Differential expression of mUltiple U1 small nuclear RNAs in oocytes and embryos of Xenopus laevis. Cell 38:681-689 Francoeur AM, Gritzmacher CA, Peebles CL, Reese RT, Thn EM (1985) Synthesis of small nuclear ribonucleoprotein particles by the malarial parasite Plasmodium Jalciparum.

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