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By Kunio Takeyasu; Kyosuke Nagata
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Extra resources for Nuclear Dynamics : Approaches from Molecular, Biochemical and Visual Biology
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J Struct Biol 147:31-41 Shopland LS, Lawrence JB (2000) Seeking common ground in nuclear complex-ity. J Cell Biol 150: Fl-4 Sleeman JE, Trinkle-Mulcahy L, Prescott AR, Ogg SC, Lamond AI (2003) Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo. J Cell Sci 116:2039-2050 Snaar S, Wiesmeijer K, Jochemsen AG, Tanke HJ, Dirks RW (2000) Mutational analysis of fibrillarin and its mobility in living human cells. J Cell Biol 151:653-662 Straight AF, Belmont AS, Robinett CC, Murray AW (1996) GFP tagging of bud-ding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.
1996), dynamic relaxation of supercoiled DNA by replication factor (Yoshimura et al. 2000a), long-range DNA loop formation in p-globin enhancer region by transcription regulators (Yoshimura et al. 2000b), and DNA end-loop formation by telomere-specific proteins (Yoshimura et al. 2004). In the 1970s, chromatin fibers were extensively studied by EM, and the higher-order arrangements of chromatin fibers such as 30-nm fibers and 300-nm loops were identified (Paulson and Laemmli 1977; Rattner and Hamkalo 1978a,b; Marsden and Laemmli 1979).
2000). These effects have a rapid time course (seconds to minutes) and are not inhibited by actinomycin D. They have been considered to reflect aldosterone acting via a high-affinity membrane receptor, distinct from the classic intracellular MR on two reasons. One is that the rapid effects have not been inhibited by spironolactone and another is that glucocorticoids do not mimic the effects of aldosterone at nanomolar concentrations. Rapid, nongenomic effects of aldosterone may also reflect actions via the classic intracellular MR.