Download Plant Viruses by Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. (auth.) PDF
By Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. (auth.)
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Extra resources for Plant Viruses
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The ability of one virus strain to inhibit the entrance of another strain of the same virus into the same plant was first demonstrated by McKinney (1929). He showed that plants infected with a strain of tobacco mosaic virus which gave rise to a light-green mosaic underwent no change in symptoms after repeated inoculations with a strain of virus causing a yellow mosaic. In 1931, Thung carried out a similar experiment with a strain of tobacco mosaic virus causing a white mosaic against the ordinary green mottling type.
5) The clarified dialyzate is centrifuged for 150 minutes at 78,000g. (6) Pellets are resuspended in 0·005 M borate buffer, pH 9, and the suspension is clarified at 5,400g for 15 minutes to remove insoluble materials and aggregates. (7) The clarified suspension is recentrifuged for 90 minutes at 105,ooog, pellets are resuspended in the same borate buffer and clarified as before. (8) Procedure number 7 is repeated. A new method of isolation which has some advantages over the methods just given has been suggested by Leberman (1966) and his technique is briefly described.
4 Isolation and Purification of Plant Viruses When investigating a possibly undescribed sap-transmissible virus, it is desirable to have some information on certain elementary properties of the virus before commencing more comprehensive studies on its purification. What is wanted is some information on its stability and concentration in the plant, because attempts at isolation are likely to be much more difficult if the virus in question is extremely labile or occurs in very low concentration. The three elementary tests usually carried out are to determine: (a) the ageing, or longevity in vitro, of the virus in extracted sap; (b) the thermal inactivation point; and (c) the dilution end-point.