By J. Colin Murrell
The excellent start line for investigating, constructing, and imposing strong isotope technologies.
- Guides researchers via uncomplicated, proven, and confirmed protocols together with DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from proposal and historical past via special technique, troubleshooting, and interpretation to optimum and destiny uses.
- Explores very important and rising functions of SIP in environmental microbiology, starting from bioremediation and gene mining to carbon monitoring and intestine microflora functionality.
- Examines explorations of additional dependent isotope labeling applied sciences similar to Raman-FISH, NanoSIMS, and isotope arrays.
- Serves as a necessary source for environmental microbiology scholars and researchers and genomics, biotechnology, and scientific microbiology execs.
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Additional resources for Stable Isotope Probing and Related Technologies
Biochem. 39:232–238. Derito, C. , and E. L. Madsen. 2009. Stable isotope probing reveals Trichosporon yeast to be active in situ in soil phenol metabolism. ISME J. 3:477–485. Derito, C. , G. M. Pumphrey, and E. L. Madsen. 2005. Use of field-based stable isotope probing to identify adapted populations and track carbon flow through a phenol-degrading soil microbial community. Appl. Environ. Microbiol. 71:7858–7865. Dumont, M. , and J. C. Murrell. 2005. Stable isotope probing—linking microbial identity to function.
Ac cessing Uncultivated Microorganisms: from the Environ ment to Organisms and Genomes and Back. ASM Press, Washington, DC. , F. Schmidt, M. H. Richnow, and C. Vogt. 2008. Protein-based stable isotope probing (protein-SIP) reveals active species within anoxic mixed cultures. ISME J. 2:1122–1133. , J. D. Neufeld, N. K. Birkeland, M. Hovland, and J. C. Murrell. 2008. Methane assimilation and trophic interaction with marine Methylomicro bium in deep-water coral reef sediment off the coast of Norway.
Cupples, A. , and G. K. Sims. 2007. Identification of in situ 2,4-dichlorophenoxyacetic acid-degrading soil microorganisms using DNA-stable isotope probing. Soil Biol. Biochem. 39:232–238. Derito, C. , and E. L. Madsen. 2009. Stable isotope probing reveals Trichosporon yeast to be active in situ in soil phenol metabolism. ISME J. 3:477–485. Derito, C. , G. M. Pumphrey, and E. L. Madsen. 2005. Use of field-based stable isotope probing to identify adapted populations and track carbon flow through a phenol-degrading soil microbial community.