By American Institute of Steel Construction

Это богато иллюстрированное издание содержит краткое описание современной технологии производства стали, а также рассказывает о её применении в различных отраслях промышленности. Основной интерес (для тех, кто не владеет английским) представляют схемы металлургических процессов и агрегатов, а также фотографии.

Другие новости автора по теме "Металлургия чёрных металлов":
Восстановление железных руд (Л. фон Богданди, Г.-Ю. Энгель) 1971 г.
Методы расчёта материального и теплового баланса коксовых печей (Ханин и др.) 1972 г.
Металлургия железа в истории цивилизации (Черноусов П.И., Мапельман В.М., Голубев О.В.) 2005 г.
Балансовая логико-статистическая модель доменного процесса (Ченцов А.В., Чесноков Ю.А., Шаврин С.В.) 2003 г.
Руководство къ металлургiи. Часть 1 (А. Добронизский) 1865 г.
Руководство къ металлургiи. Часть 2 ( Д. Перси, А. Добронизский) 1869 г.
Основы металлургического производства (чёрная металлургия). Учебник для СПТУ (Бабич В.К. и др.) 1988 г.
Состояние и перспективы бездоменной металлургии железа (Курунов И.Ф., Савчук Н.А.) 2002 г.
Металлургия железа (Юсфин Ю.С., Пашков Н.Ф.) 2007 г.
Переработка шлаков и безотходная технология в металлургии (М.И Панфилов и др.) 1987 г.
Производство чугуна. Краткое руководство доменной плавки. Изданiе 2-е (де Билли) 1900 г.
Агломерация рудных материалов (Коротич В.И. и др.) 2003 г.
Металлургiя чугуна (Валериусъ) 1862 г.

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5. Spin the tube at 13,000 rpm for 2 min. Transfer the aqueous phase to a Wizard SV minicolumn (included in Wizard Plus SV Minipreps DNA Purification System, Promega, Cat# A1460), place the minicolumn in a microcentrifuge tube, and spin at 13,000 rpm for 2 min. 6. Transfer the aqueous phase (∼450 μL) to a new microcentrifuge tube. 5 μL of glycogen and 1 mL of –20 ºC ethanol. Invert the tube several times to mix well. Incubate at –20 ºC for 1 h to overnight. 7. Spin at 13,000 rpm for 5 min at 4 ºC.

When performing radioactive experiments, always take care to avoid contaminating the environment, and protect yourself and people around you from exposure to the radioactive materials. 7. To minimize RNase contamination, all solutions and buffers used in these experiments should be made with DEPC-treated water. Always wear gloves, and use RNase-free tips and reagents designated to the RNA bench. 8. When preparing radiolabeled dsRNA or pre-miRNA substrates, it is best to verify the quality of the radiolabeled probes by running 1 μL of probe on a denaturing PAGE prior to performing the actual assays.

Nature 418, 244–251. 2. Bartel, D. P. (2004). MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116,281–297. 3. , Kolb, F. , and Pillai, R. S. (2005). Posttranscriptional gene silencing by siRNAs and miRNAs. Curr. Opin. Struct. Biol. 15, 331–341. 4. Sontheimer, E. , and Carthew, R. W. (2005). Silence from within: Endogenous siRNAs and miRNAs. Cell 122, 9–12. 5. , and Slack, F. J. (2006). Oncomirs—MicroRNAs with a role in cancer. Nat. Rev. Cancer 6, 259–269. 6. Plasterk, R. H. (2006).

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