By Carlos Augusto Rosa, Gabor Peter
Within the previous couple of a long time an increasing number of yeast habitats were explored, spanning chilly climates to tropical areas and dry deserts to rainforests. accordingly, a wide physique of ecological info has been amassed and the variety of identified yeast species has elevated swiftly. This e-book presents an outline of the biodiversity of yeasts in numerous habitats. fresh advances accomplished through the applying of molecular organic equipment within the box of yeast taxonomy and ecology also are integrated within the publication. anyplace attainable, the interplay among yeasts and the encompassing atmosphere is mentioned.
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Extra resources for Biodiversity and Ecophysiology of Yeasts (The Yeast Handbook)
Gov Internet address • National Collection of Yeast Cultures Coleccion Espanola de Cultivos Tipo Japan Collection of Microorganisms, Bioresource Center All-Russian Collection of Microorganisms Mycotheque de l’Universite catholique de Louvain American Type Culture Collection Labatt Culture Collection, Technology Development Culture Collection of Industrial Microorganisms National Center For Agricultural Utilization Research Centraalbureau voor Schimmelcultures University of California Davis, Herman J.
J. -A. Lachance, among many others, also did pioneering work by collecting previously ignored or less sampled areas or substrates like cacti or insects. From our experience of fieldtrips to South America, Africa and Asia, we estimate that roughly 50% of the material collected, which mainly originated from flowers, represented new species. Strikingly, a number of isolates originating from different continents belonged to the exact same new species (not yet published but available from the CBS CC).
The application of these techniques to fungal ecology was reviewed by Anderson and Cairney (2004). By necessity, all of these techniques must be confirmed by sequence analysis. Therefore, the bias and research concentration in our laboratories has been directed to sequence-based identification methods. PCR-based species-specific primers represent the least expensive and easiest method for identification of small numbers (one to ten) of taxa (Chapman et al. 2003). Multiplex PCR of larger numbers of primers in a single reaction is difficult owing to various factors, such as formation of primer dimers and differences in temperature requirements for primer hybridizations.