By S. Yamamoto, T. Yoshimoto, H. Suzuki, N. Ueda, K. Natsui, Y. Takahashi, T. Maruyama (auth.), Kenneth V. Honn, Lawrence J. Marnett, Santosh Nigam, Thomas L. Walden Jr. (eds.)
This quantity includes the complaints of the 1st overseas convention on Eicosanoids and different Bioactive Lipids in melanoma and Radiation damage held in Detroit, Michigan on October 11-14, 1989. this system consisted of eighty three oral and 29 poster shows, seventy four of that are incorporated in those lawsuits. the main sponsors of the convention have been the military Radiobiology examine Institute, positioned in Bethesda, Maryland, the Radiation Oncology examine and improvement heart of the Gershenson Radiation Oncology middle, Harper medical institution in Detroit, Michigan, and Schering AG of West Germany. Eighteen different businesses supplied extra aid. The convention used to be detailed in its try to hyperlink the eicosanoid and lipid researchers within the radiobiology and melanoma disciplines. the various roles that eicosanoids and different bioactive lipids play in those organic phenomena together with the participation of lipid oxidation in conversion of procarcinogens, confident and damaging modulation of tumor progress, immunomodulation, tissue harm, and but defense and enhancement of melanoma remedy, necessitated clinical interplay to tackle and comprehend those complicated and infrequently contradictory observations. The good fortune of this attempt is mirrored not just via those lawsuits, but in addition in the course of the selection to proceed the convention sequence with a moment assembly to be held in Berlin among September 17-21, 1991.
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Extra info for Eicosanoids and Other Bioactive Lipids in Cancer and Radiation Injury: Proceedings of the 1st International Conference October 11–14, 1989 Detroit, Michigan USA
In these organs, parenchymal cells were not stained significantly, and the positively stained cells were resident mast cells or infiltrating leukocytes (18). Therefore, we subjected anterior pituitary to immunohistological examination to find out whether or not the 12-lipoxygenase detected by the enzyme immunoassay was attributed to the leukocytes. When porcine anterior pituitary was stained by the avidinbiotin method, certain parenchymal cells rather than infiltrating leukocytes were positively stained.
For screening the 12-lipoxygenase eDNA, two oligonucleotide probes were prepared on the basis of the amino acid sequence of a peptide obtained by digestion of the purified 12Iipoxygenase. Transformed E. coli cells were screened by the use of the two oligonucleotide probes. The nucleotide sequence of the 12-lipoxygenase eDNA was determined by the dideoxynucleotide chain termination method of Sanger. The amino acid sequence deduced from the nucleotide sequence indicated that the enzyme protein was presumably composed of 662 amino acid residues.
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