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Battley, E. H. (1964). Antonie van Leeuwenhoek, 30, 81-96. Brown, C. , and Rose, A. H. J. , 97,261-272. Brown, C. , and Rose, A. H. J. , 98, in press. , and I h n t z , J. A. (1954). Ann. N . Y . Acud. , 58,1303-1310. 38 J. W. PATCHING AND A. H. , and Wallach, D. F. H. (1968). In “Biological Membranes” (Ed, D. Chapman), pp. 125-202. Academic Press, London. Collins, C. H. (1967). “MicrobiologicalMethods”, p. 53. Butterworths, London. Deutsch, W. W. (1962). Rev. scient. , 33, 249-250. Dimmick, R.

These examples emphasize that it is necessary to distinguish between effects of temperature on growth and on individual metabolic activities of microorganisms. 11. CONTROL O F TEMPERATURE I N MICROBIOLOGICAL STUDIES A. Maintenance of stock cultures It is common practice to store stock cultures of micro-organisms on, or in, solidified nutrient media at a temperature near or below the minimum for growth of the organisms on that medium. Under these conditions, further growth of the organisms is prevented or severely restricted, thereby ensuring that metabolic waste products do not accumulate in high concentrations in the culture.

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